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SARS-CoV-2 (2019-nCoV) Spike Protein (S1+S2 ECD, His tag)上海惠诚生物现货供应,联系电话:17715331663 QQ:2303607288
| Chemical Name |
SARS-CoV-2 (2019-nCoV) Spike Protein (S1+S2 ECD, His tag)
|
| Qty 1 |
1mg
|
| Qty 2 |
10mg
|
| Storage condition |
-20℃ to -80℃
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| References |
SARS-CoV-2 (2019-nCoV) Spike Protein (S1+S2 ECD, His tag): 产品信息纯度> 90 % as determined by SDS-PAGE.
内毒素< 1.0 EU per μg protein as determined by the LAL method.
生物活性The 2019-nCoV Spike Protein (S1+S2 ECD, His tag) can bind with Human ACE2 in functional ELISA assay.
蛋白构建A DNA sequence encoding the NCP-CoV(2019-nCoV) Spike Protein (S1+S2 ECD) was expressed with a polyhistidine tag at the C-terminus.
表达宿主Baculovirus-Insect Cells
种属2019-nCoV
预测 N 端Val
分子量The recombinant NCP-CoV(2019-nCoV) Spike Protein (S1+S2 ECD, His tag) consists of 1209 amino acids and predicts a molecular mass of 134.36 kDa.
缓冲液
Lyophilized from sterile 20 mM Tris, 300 mM NaCl, 10 % glycerol, pH 8.0.
Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
运输方式
一般情况下，重组蛋白以冻干粉的形式提供，并在常温下运输。
重组蛋白的散装包装作为冷冻液体提供。除非客户另有要求，否则我们会用蓝冰发货。
稳定性 & 储存条件
样品在-20℃至-80℃条件下，自收到之日起12个月内保持稳定
储存于-20℃至-80℃无菌条件下。建议对蛋白质进行校正，以达到的贮藏效果。避免重复的冻融循环。
SARS-CoV-2 (2019-nCoV) Spike Protein (S1+S2 ECD, His tag): 别称coronavirus spike Protein, 2019-nCoV; cov spike Protein, 2019-nCoV; ncov RBD Protein, 2019-nCoV; ncov s1 Protein, 2019-nCoV; ncov s2 Protein, 2019-nCoV; ncov spike Protein, 2019-nCoV; NCP-CoV RBD Protein, 2019-nCoV; NCP-CoV s1 Protein, 2019-nCoV; NCP-CoV s2 Protein, 2019-nCoV; NCP-CoV Spike Protein, 2019-nCoV; novel coronavirus RBD Protein, 2019-nCoV; novel coronavirus s1 Protein, 2019-nCoV; novel coronavirus s2 Protein, 2019-nCoV; novel coronavirus spike Protein, 2019-nCoV; RBD Protein, 2019-nCoV; S1 Protein, 2019-nCoV; S2 Protein, 2019-nCoV; Spike RBD Protein, 2019-nCoV
CoV spike 背景信息冠状病毒的糖蛋白突起只会与宿主细胞的某些受体结合。已知的受体结合S1是ACE2，血管紧张素转换酶2;DPP4, dipeptidyl peptidase-4;比例导引,氨肽酶N;CEACAM，癌胚抗原相关细胞粘附分子1;新航,唾液酸;o - acsia, o -乙酰化唾液酸。该峰对于宿主特异性和病毒感染性都是至关重要的。术语“peplomer”通常指的是病毒表面的一组异种蛋白质，它们共同发挥作用。已知冠状病毒的糖蛋白峰在感染过程开始时是病毒与宿主细胞结合的关键。据报道，2019-nCoV可通过与人ACE2受体相互作用感染人呼吸道上皮细胞。穗蛋白是一个大的I型跨膜蛋白，包含两个亚基，S1和S2。S1主要包含一个受体结合域(RBD)， RBD负责识别细胞表面受体。S2包含膜融合所需的基本元素。S蛋白在诱导中和抗体和t细胞反应以及免疫保护中起关键作用。其主要功能概括为:介导受体结合和膜融合;定义宿主的范围和病毒的特异性;与中和抗体结合的主要成分;疫苗设计的关键目标;可通过受体结合域(RBD)的基因重组或突变在不同宿主间传播，导致较高的死亡率。
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